Hplc Column Care And Maintenance
The column is a key factor in HPLC chromatography separation hence preventive care and maintenance of HPLC column required for better performance and increasing span life of a column. This course is ideal for those chromatographers involved in practical HPLCUHPLC who want to ensure longer column lifetime and greater reproducibility.
Hplc Column Cleaning And Regeneration
Each column is a precision instrument which if cared for properly will provide significant performance lifetime and repeatability.
Hplc column care and maintenance. If you are unsatisfied for any reason please give your Phenomenex Technical Represen-tative a call. Care and Maintenance of HPLC Columns. Avoid using a viscous buffer.
In the early years the Chromatography work carried out by the glass column with the diameter 01 to 05 cm and length is 50 to 500 cm now a day HPLC columns are made of stainless steel. The flow direction is always marked on the analytical column. Suppose Acetonitrile is used in mobile phase preparation then the column can be washed with Acetonitrile.
General HPLC Column Care For mobile Phase without buffer Salts wash the column with the solvent which was used for the preparation of the mobile phase in that particular analysis using a flow rate of 1 ml per min for 30 min eg. The column is packed with porous particles and its made of polymer and is bounded by a thin layer of silica and polystyrene. This depends mainly on Mobile Phase and Flow Rate and also the nature of the sample being analysed.
This depends mainly on Mobile Phase and Flow Rate and also the nature of the sample being analysed. Do not overload the HPLC column resulting in broad peaks of analytes. Always maintain the pH of the buffermobile phase in the range of 20 to 95 pH.
Care should again be taken that the HPLC column has been engineered to accommodate highly aqueous solvents traditional alkyl chain media can be pro ne to phase collapse in low organic composition solvents mixes for example at. HPLC column care Maximum lifetime and performance for an HPLC column is best achieved through a proper program of care and maintenance. Make use of only HPLC grade solvents and the freshly prepared buffer solution to reduce any bacterial growth in the mobile phase.
In HPLC analysis every chromatographer know the importance of column because of the theoretical plate number resolution tailing factor peak symmetry and system suitability depend on. Introduce the most commonly observed column related problems in HPLC. HPLC column care In general HPLC column that is performing well offers the following characteristics.
PEOPLE ALSO READ. Performance testing of the columnIt is suggested that every column performance should be tested during the arrival and periodic intervals HPLC solvents. HPLC column care Maximum lifetime and performance for an HPLC column is best achieved through a proper program of care and maintenance.
If a column oven is available then maintain column temperature and also maintain the room temperature. Valuable preventative column care including mobile phase pH temperature column storage filters and guards will be discussed. Phosphate buffer concentrations should be less than 20 mM.
Propose preventative maintenance and method developmentoptimization approaches to minimize HPLC column problems and increase column lifetimes. HPLC Column Care Guide Phenomenex UHPLC HPLC SPE and GC. Rinse columnRinse column remove detector from flow pathremove detector from flow path Eliminate column contamination and plugged packing higgg ph molecular wei ghtadsorbed com pounds precipitate from sample or buffer Back flush column may clear plugged column inlet frit Change column inlet frit or discard column.
Guidelines demand less exposure to organic solvents. This column care guide is designed with these factors in mind allowing you to extract the utmost from your HPLC column. Column Warranties Phenomenex HPLC columns are warranted to meet the stated performance and quality and to be free of defects in material and workmanship.
This course is ideal for those chromatographers involved in practical HPLCUHPLC who want to ensure longer column lifetime and greater reproducibility in their methods. Well do our best to solve the problem to your satisfaction. Early in our history our coatings were developed to offer solutions for difficult process sampling and GC sampling applications.
Valuable preventative column care including mobile phase pH temperature column storage filters and guards will be discussed. Nano LC Capillary LC Analytical LC Prep LC Chip LC. Explore the reasons for these column problems.
To ensure the columns life maximum keep the pH of the mobile phase between 2 to 95 and always filter with a 045-micron filter. Thats why HPLC column care and robust sampling system maintenance is important to achieving reliable HPLC test results and bio-inertness. HPLC column care Physical Changes Chemical Changes Preventive measures Corrective measures.
It is designed to help alleviate problems before they occur and covers a range of topics.
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